Peroxydisulfate-Driven Reductive Dechlorination as Affected by Soil Constituents: Free Radical Formation and Conversion.

We report a previously unrecognized but efficient reductive degradation pathway in peroxydisulfate (PDS)-driven soil remediation. With supplements of naturally occurring low-molecular-weight organic acids (LMWOAs) in anaerobic biochar-activated PDS systems, degradation rates of 12 γ-hexachlorocyclohexanes (HCH)-spiked soils boosted from 40% without LMWOAs to a maximum of 99% with 1 mM malic acid. Structural analysis revealed that an increase in α-hydroxyl groups and a diminution in pKa1 values of LMWOAs facilitated the formation of reductive carboxyl anion radicals (COO•-) via electrophilic attack by SO4•-/•OH. Furthermore, degradation kinetics were strongly correlated with soil organic matter (SOM) contents than iron minerals. Combining a newly developed in situ fluorescence detector of reductive radicals with quenching experiments, we showed that for soils with high, medium, and low SOM contents, dominant reactive species switched from singlet oxygen/semiquinone radicals to SO4•-/•OH and then to COO•- (contribution increased from 30.8 to 66.7%), yielding superior HCH degradation. Validation experiments using SOM model compounds highlighted critical roles of redox-active moieties, such as phenolic - OH and quinones, in radical formation and conversion. Our study provides insights into environmental behaviors related to radical activation of persulfate in a broader soil horizon and inspiration for more advanced reduction technologies.

Preparation and physicochemical properties characterization of hesperetin-grafted pectin conjugate.

Different ratios of hesperetin (HT) were successfully grafted onto pectin from basic water (PB) molecules via free radical-induced reaction. The structure of PB-HT conjugates was characterized by ultraviolet spectroscopy, infrared spectroscopy, X-ray diffraction and scanning electron microscopy. Results indicated that HT was successfully grafted onto pectin molecules, and PB-HT-0.5 showed the highest HT content (103.18 ± 2.76 mg/g). Thermogravimetric analysis indicated that HT crystals showed good thermal resistance and could improve the thermal stability of PB-HT conjugates. Additionally, PB-HT conjugates showed good cytocompatibility and blood compatibility. This study provides a novel and efficient method to synthesize hesperetin-grafted pectin conjugate, which showed potential application in the fields of functional foods in the future.

Goji berry leaf exerts a comparable effect against colitis and microbiota dysbiosis to its fruit in dextran-sulfate-sodium-treated mice.

Goji berry and mulberry are both popular berries with anti-colitis effects, but their leaves have received less attention. In this study, the anti-colitis effects of goji berry leaf and mulberry leaf were investigated in dextran-sulfate-sodium-induced colitis C57BL/6N mice compared with their fruits. Goji berry leaf and goji berry reduced colitic symptoms and ameliorated tissue damage, while mulberry leaf did not. ELISA and western blotting analysis suggested that goji berry showed the best performance in inhibiting the overproduction of pro-inflammatory cytokines (TNF-α, IL-6 and IL-10) and improving damaged colonic barrier (occludin and claudin-1). Besides, goji berry leaf and goji berry reversed the gut microbiota dysbiosis by increasing the abundance of beneficial bacteria like Bifidobacterium and Muribaculaceae, and decreasing the abundance of harmful bacteria like Bilophila and Lachnoclostridium. Goji berry, mulberry and goji berry leaf could restore acetate, propionate, butyrate and valerate to ameliorate inflammation, while mulberry leaf could not restore butyrate. To the best of our knowledge, this is the first report on the comparison of the anti-colitis effects of goji berry leaf, mulberry leaf and their fruits, which is meaningful for the rational utilization of goji berry leaf as a functional food.

RG-I Domain Matters to the In Vitro Fermentation Characteristics of Pectic Polysaccharides Recycled from Citrus Canning Processing Water.

Canned citrus is a major citrus product that is popular around the world. However, the canning process discharges large amounts of high-chemical oxygen demand wastewater, which contains many functional polysaccharides. Herein, we recovered three different pectic polysaccharides from citrus canning processing water and evaluated their prebiotic potential as well as the relationship between the RG-I domain and fermentation characteristics using an in vitro human fecal batch fermentation model. Structural analysis showed a large difference among the three pectic polysaccharides in the proportion of the rhamnogalacturonan-I (RG-I) domain. Additionally, the fermentation results showed that the RG-I domain was significantly related to pectic polysaccharides' fermentation characteristics, especially in terms of short-chain fatty acid generation and modulation of gut microbiota. The pectins with a high proportion of the RG-I domain performed better in acetate, propionate, and butyrate production. It was also found that Bacteroides, Phascolarctobacterium, and Bifidobacterium are the main bacteria participating in their degradation. Furthermore, the relative abundance of Eubacterium_eligens_group and Monoglobus was positively correlated with the proportion of the RG-I domain. This study emphasizes the beneficial effects of pectic polysaccharides recovered from citrus processing and the roles of the RG-I domain in their fermentation characteristics. This study also provides a strategy for food factories to realize green production and value addition.

RGI-Type Pectic Polysaccharides Modulate Gut Microbiota in a Molecular Weight-Dependent Manner In Vitro.

In this study, the fermentation characteristics of high rhamnogalacturonan I pectic polysaccharides (RGI) and free-radical degraded RGI (DRGI) were evaluated by a human fecal batch-fermentation model, and their structural properties were also investigated. As a result, the Mw of RGI decreased from 246.8 to 11.6 kDa, and the branches were broken dramatically. Fermentation showed that RGI degraded faster and produced more acetate and propionate than DRGI. Both of them reduced the Firmicutes/Bacteroidetes ratio and promoted the development of Bacteroides, Bifidobacterium, and Lactobacillus, bringing benefits to the gut ecosystem. However, the composition and metabolic pathways of the microbiota in RGI and DRGI were different. Most of the dominant bacteria of RGI (such as [Eubacterium]_eligens_group) participated in carbohydrate utilization, leading to better performance in glucolipid metabolism and energy metabolism. This work elucidated that large molecular weight matters in the gut microbiota modulatory effect of RGI-type pectic polysaccharides in vitro.

Alternating electric field enables hyperthermophilic composting of organic solid wastes.

Hyperthermophilic composting (HTC) achieves compost temperatures above 80 °C, usually depending on the inoculated hyperthermophilic bacteria, which has been well used in full-scale plants. However, the scarcity of hyperthermophilic bacteria and the high cultivation cost hinder the development of HTC. Recently, a direct-current electric field applied on conventional aerobic composting raised compost temperature to 70-75 °C, but gradient moisture distribution under the action of the direct-current electric field affected microbial metabolic heat and limited the temperature rise. Herein the effects of alternating electric field (AEF) promoting a uniform water distribution and further raising the temperature to achieve HTC were investigated. Our results demonstrated that AEF raised the compost temperature to 90 °C, and the period with temperatures above 80 °C lasted 4 days. The physicochemical properties and maturity index showed that the AEF improved the biodegradation and humification of organic matter due to the generation of metabolic heat. The AEF enriched thermophilic bacteria (Ureibacillus: by 52.36% on day 3; Navibacillus: by 46.54% on day 41). A techno-economic analysis indicated that the proposed approach with the AEF had a cost advantage over HTC with the inoculation of hyperthermophilic bacteria. Therefore, the AEF composting system represents a novel and applicable strategy for HTC.

Moisture migration driven by the electric field causes the directional differentiation of compost maturity.

Electric field-assisted aerobic composting (EAC) has been recently believed as a novel and effective process for the resource utilization of organic solid waste. However, the effect of electric field in composting process needs to be further clarified. Herein, moisture migration and compost maturity along electric-field-direction (from anode to cathode) in EAC was first to be explored. It was found that moisture content and compost maturity changed regularly from anode to cathode. At the end of composting, the moisture content of S3 (cathodic zone) was 30% and 62% higher than that of S2 (middle zone) and S1 (anodic zone), respectively. The germination index (a key parameter for compost maturity) in S3 (138.92%) was significantly higher than that of S2 (104.98%) and S1 (84.45%). However, temperatures in S3 were lower than that of S1 and S2, indicating the moisture content played a more important role than temperature for compost maturity in EAC. Furthermore, the microbial activities in S3 were also higher than that of S1 and S2, supporting the trend of compost maturity. This pioneering study demonstrates the electric field can drive moisture gradient migration to control the directional differentiation of compost maturity, showing a great application potential in aerobic composting.

In-situ electrolytic oxygen is a feasible replacement for conventional aeration during aerobic composting.

Aerobic composting is an effective recycling method for the disposal and resource utilization of organic solid waste. However, the inappropriate aeration mode used during conventional aerobic composting (CAC) often results in low oxygen utilization efficiency and loss of temperature, which further leads to a long maturation period and large odorous gas (NH3) pollution. Herein, a novel electrolytic oxygen aerobic composting (EOAC) process was invented first using in-situ oxygen generation for aeration by the electrolysis of water in compost. Our results demonstrated that the germination index (GI) significantly increased during EOAC, and the maturation time of compost was shortened by nearly 50% during EOAC compared to CAC, indicating higher oxygen utilization efficiency during EOAC. Meanwhile, NH3 emissions, N2O emissions, and nitrogen loss during the EOAC process decreased by 61%, 46%, and 21%, respectively, compared to CAC. The total relative abundance of thermophilic and electroactive bacteria during EOAC increased remarkably. EOAC inhibited ammoniation, nitrification, and denitrification, and weakened N-associated functional genes. A techno-economic analysis indicated that EOAC had greater technical superiority and cost advantages compared to CAC. This study represents proof-of-principle for EOAC and suggests that in-situ electrolytic oxygen is a feasible replacement for conventional aeration during aerobic composting.

Insight into the synergistic effects of conductive biochar for accelerating maturation during electric field-assisted aerobic composting.

Electric field-assisted aerobic composting (EAC) has been considered as a novel and effective process for enhancing compost maturation. However, the poor conductivity of compost piles affects the efficiency and applicability of EAC. Thus, this study aims to examine how conductive biochar affects compost maturation in biochar-added electric field-assisted aerobic composting (b-EAC). Our results demonstrated that the germination index and humus index significantly increased, and the compost maturation time was shortened by nearly 25% during b-EAC compared to EAC. The total oxygen utilization rate and total relative abundance of electroactive bacteria during b-EAC increased by approximately two and three times those in EAC, respectively. These findings indicated that the addition of conductive biochar has a synergistic effect which facilitated oxygen utilization by reducing resistance and accelerating electron transfer. Therefore, the addition of conductive biochar is proved to be an effective and applicable strategy for optimizing the efficiency of EAC.

Alternating magnetic field mitigates N2O emission during the aerobic composting of chicken manure.

Nitrous oxide (N2O) emission is an environmental problem related to composting. Recently, the electric field-assisted aerobic composting process has been found to be effective for enhancing compost maturity and mitigating N2O emission. However, the insertion of electrodes into the compost pile causes electrode erosion and inconvenience in practical operation. In this study, a novel alternating magnetic field-assisted aerobic composting (AMFAC) process was tested by applying an alternating magnetic field (AMF) to a conventional aerobic composting (CAC) process. The total N2O emission of the AMFAC process was reduced by 39.8% as compared with that of the CAC process. Furthermore, the results demonstrate that the AMF weakened the expressions of the amoA, narG, and nirS functional genes (the maximum reductions were 96%, 83.7%, and 95.5%, respectively), whereas it enhanced the expression of the nosZ functional gene by a maximum factor of 36.5 as compared with that in CAC. A correlation analysis revealed that the nitrification and denitrification processes for N2O emission were suppressed in AMFAC, the main source of N2O emission of which was denitrification. The findings imply that AMFAC is an effective strategy for the reduction of N2O emission during aerobic composting.

Low shear stress induced vascular endothelial cell pyroptosis by TET2/SDHB/ROS pathway.

Vascular endothelial cell (VEC) inflammation induced by low shear stress plays key roles in the initiation and progression of atherosclerosis (As). Pyroptosis is a form of inflammatory programmed cell death that is critical for As. However, the effect of low shear stress on VEC pyroptosis and the underlying mechanisms were not clear. Here we show that low shear stress promoted VEC pyroptosis and reduced the expression of Ten-Eleven Translocation 2 (TET2) methylcytosine dioxygenase. Loss of TET2 resulted in the upregulation of the expression and activity of mitochondrial respiratory complex II subunit succinate dehydrogenase B (SDHB) by decreasing the recruitment of histone deacetylase 2, independent of DNA demethylation modification. The overexpression of SDHB mediated mitochondrial injury and increased the production of reactive oxygen species (ROS). The administration of ROS scavenger NAC alleviated VEC pyroptosis induced by SDHB overexpression and TET2 shRNA. These findings show that low shear stress induced endothelial cell pyroptosis through the TET2/SDHB/ROS pathway and offer new insights into As.

Use of an in situ thermoelectric generator for electric field-assisted aerobic composting.

Electric field-assisted aerobic composting (EAAC) is a simple and effective process. To further improve the EAAC process and make good use of waste heat during composting, in this study, we designed an in situ thermoelectric generator using thermoelectric sheets and applied it for EAAC. The findings show that the voltage generated was 8.8-18.6 V, and the maximum power was over 7 W. A direct current-to-direct current (DC-DC) voltage converter was used to stabilize the output at 6.0 V. Self-powered EAAC (sp-EAAC) enhanced compost maturity compared to conventional aerobic composting (CAC). The germination index reached 118% and 88% in sp-EAAC and CAC, respectively, at the end of composting. This work verified that the temperature gap between compost and the environment could be used for the EAAC process, opening a new way to recover waste heat during aerobic composting and accelerate compost maturity.

Essential Role of Nonessential Amino Acid Glutamine in Atherosclerotic Cardiovascular Disease.

Atherosclerosis is a major disease that seriously harms human health and is known as the "number one killer" in developed countries and the leading cause of death worldwide. Glutamine is the most abundant nonessential amino acid in the human blood that has multifaceted effects on the body. Recent studies showed that glutamine is negatively corrected with the progression of atherosclerotic lesions. In this review, we focused on the relationship of glutamine with macrophage polarization, nitrification stress, oxidative stress injury, myocardial ischemia-reperfusion injury, and therapeutic angiogenesis to review its roles in atherosclerotic cardiovascular disease.

In situ probing behaviors of single LiNiO2 nanoparticles by merging CAFM and AM-FM techniques.

Probing single active nanoparticles of Li-ion battery electrodes is challenging but important to reveal their behaviors including morphology, mechanical properties and electrochemical reactions with an electrolyte. In this work, we in situ investigated voltage-induced behaviors of single LiNiO2 nanoparticles by merging conductive atomic force microscopy (CAFM) and amplitude modulation-frequency modulation (AM-FM) techniques. The former was used to apply a voltage between a selected single nanoparticle and a substrate through its tip, while the latter was done for imaging. Evolution in the morphology and stiffness of the nanoparticles induced by different voltages under air and dried argon atmospheres was tracked, respectively. The evolution mechanisms related to electrochemical reactions were discussed in detail. These results suggest that the merged techniques would provide an indirect and effective approach to study the behaviors and electrochemical reactions of electrode materials on the nanometer scale and even single nanoparticles.

Multi-characterization of LiCoO2 cathode films using advanced AFM-based techniques with high resolution.

ABSTARCT: The thin film Li-ion batteries have been extensively used in micro-electronic devices due to their miniaturization, high capacity density and environmental friendliness, etc. In order to further prolong the lifetime of the film batteries, one of important tasks is to explore the aging mechanisms of the cathode films. In this paper, we especially focused on the multi-characterization of the LiCoO2 film in nanoscale, which is carried out by combining advanced AFM-based techniques with capacity measurement. The surface morphology, contact stiffness as well as surface potential were measured by amplitude modulation-frequency modulation (AM-FM) and kelvin probe force microscope (KPFM), respectively. Remarkable changes after different numbers of charge/discharge cycling were observed and the intrinsic reasons of them were discussed in detail. To acknowledge the relationship with these microscopic changes, the macro-capacity of the thin films was also measured by the galvanostatic charge/discharge method. These comprehensive results would provide a deep insight into the fading mechanism of the cathode film, being helpful for the design and selection of the cathode film materials for high performance batteries.

Temperature-Dependent Lithium-Ion Diffusion and Activation Energy of Li1.2Co0.13Ni0.13Mn0.54O2 Thin-Film Cathode at Nanoscale by Using Electrochemical Strain Microscopy.

This paper presents the in situ mapping of temperature-dependent lithium-ion diffusion at the nanometer level in thin film Li1.2Co0.13Ni0.13Mn0.54O2 cathode using electrochemical strain microscopy. The thin-film Li1.2Co0.13Ni0.13Mn0.54O2 cathode exhibits higher lithium-ion diffusivities with increasing temperature, which explains the higher capacity observed in the lithium-ion batteries with a Li-rich cathode at elevated temperature. In addition, the activation energy for lithium-ion diffusion can be extracted in an Arrhenius-type plot at the level of grain structure with the assumption that the ionic movement is diffusion controlled. Compared with the grain interiors, the grain boundaries show relatively lower activation energy; hence, it is the preferred diffusion path for lithium ions. This study has bridged the gap between atomistic calculations and traditional macroscopic experiments, showing direct evidence as well as mechanisms for ionic diffusion for Li-rich cathode material.

In situ Electrochemical-AFM Study of LiFePO4 Thin Film in Aqueous Electrolyte.

Lithium-ion (Li-ion) batteries have been widely used in various kinds of electronic devices in our daily life. The use of aqueous electrolyte in Li-ion battery would be an alternative way to develop low cost and environmentally friendly batteries. In this paper, the lithium iron phosphate (LiFePO4) thin film cathode for the aqueous rechargeable Li-ion battery is prepared by radio frequency magnetron sputtering deposition method. The XRD, SEM, and AFM results show that the film is composed of LiFePO4 grains with olivine structure and the average size of 100 nm. Charge-discharge measurements at current density of 10 µAh cm(-2) between 0 and 1 V show that the LiFePO4 thin film electrode is able to deliver an initial discharge capacity of 113 mAh g(-1). Specially, the morphological changes of the LiFePO4 film electrode during charge and discharge processes were investigated in aqueous environment by in situ EC-AFM, which is combined AFM with chronopotentiometry method. The changes in grain area are measured, and the results show that the size of the grains decreases and increases during the charge and discharge, respectively; the relevant mechanism is discussed.